Journal: STAR Protocols

Article Title: Generation, expansion, and drug treatment of hematopoietic progenitor cells derived from human iPSCs

doi: 10.1016/j.xpro.2022.101400

Figure Lengend Snippet:

Article Snippet: Sl/Sl (IL-3, SCF) Cell Line , STEMCELL Technologies , Cat# 00302.

Techniques: Recombinant, Knock-Out, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

doi: 10.3390/ijms23020869

Figure Lengend Snippet: Critical micelle concentration (CMC) values for TX-100 and SL-11W based on fluorescence spectroscopy measurements. Peak wavelength of fluorescent probe (1-pyrenecarboxaldehyde) as a function of test compound concentration in PBS for ( A ) TX-100 and ( B ) SL-11W. The chemical structure of each detergent molecule is presented above the corresponding graph. The n values for TX-100 and SL-11W are 9–10 and 1–1.25, respectively, and were defined accordingly to be 9.5 and 1 for molecular weight calculations. Each data point represents the mean ± standard deviation from four technical replicates. The highest compound concentration at which there is no peak shift (relative to the baseline) is defined as the CMC value and indicated by shading.

Article Snippet: Triton X-100 (TX-100) and 1-pyrenecarboxaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Simulsol SL 11W (SL-11W) was obtained from Seppic Inc. (Fairfield, NJ, USA).

Techniques: Concentration Assay, Fluorescence, Spectroscopy, Molecular Weight, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

doi: 10.3390/ijms23020869

Figure Lengend Snippet: QCM-D tracking of the real-time interactions between SL-11W detergent and a DOPC SLB platform. Time-resolved ∆f (blue line with circles) and ∆D (red line with squares) shifts are presented as a function of time for SLB treatment with ( A ) 4000, ( B ) 2000, ( C ) 1000, ( D ) 500, ( E ) 250, or ( F ) 125 μM SL-11W. The baseline values at t = 0 min correspond to a DOPC SLB adlayer and arrows 1 and 2 denote the start of compound addition and buffer washing, respectively.

Article Snippet: Triton X-100 (TX-100) and 1-pyrenecarboxaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Simulsol SL 11W (SL-11W) was obtained from Seppic Inc. (Fairfield, NJ, USA).

Techniques: QCM-D

Journal: International Journal of Molecular Sciences

Article Title: Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

doi: 10.3390/ijms23020869

Figure Lengend Snippet: Comparison of QCM-D measurement responses for TX-100 and SL-11W interactions with SLB platform. Representative QCM-D measurement signals and corresponding data points that were used to define the baseline (arrow 1) and to calculate the maximum (arrow 2) and final (arrow 3) responses due to ( A ) TX-100 and ( B ) SL-11W detergent interactions with the SLB platform. Maximum ∆f (top panel) and ∆D (bottom panel) shifts corresponding to transient interaction processes occurring between ( C ) TX-100 or ( D ) SL-11W detergents and SLB platform. Final ∆f (top panel) and ∆D (bottom panel) shifts post-treatment corresponding to membrane-disruptive effects of ( E ) TX-100 and ( F ) SL-11W detergents on SLB platform after the last buffer washing step. The dashed lines denote the typical measurement values for a complete SLB adlayer. Shaded regions indicate concentration ranges in which the compounds are in the micellar state and the percentage values listed in ( E , F ) correspond to SLB removal fractions according to the Sauerbrey equation. Data are reported as mean ± standard deviation from four measurements.

Article Snippet: Triton X-100 (TX-100) and 1-pyrenecarboxaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Simulsol SL 11W (SL-11W) was obtained from Seppic Inc. (Fairfield, NJ, USA).

Techniques: Comparison, QCM-D, Membrane, Concentration Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Supported Lipid Bilayer Platform for Characterizing the Membrane-Disruptive Behaviors of Triton X-100 and Potential Detergent Replacements

doi: 10.3390/ijms23020869

Figure Lengend Snippet: Schematic summary of TX-100 and SL-11W detergent interactions with DOCP SLB platform and proposed morphological changes based on QCM-D data. The DOPC phospholipid and TX-100 and SL-11W detergent molecules are represented by gray, orange, and magenta headgroup colors, respectively. Illustrative steps are shown for ( A ) TX-100 that induces rapid membrane solubilization starting from compound addition and resulting in complete lipid bilayer removal, and ( B ) SL-11W that induces membrane budding upon compound addition but does not cause membrane solubilization as indicated by final QCM-D measurement responses post-washing. Note that the spherical structures in AI and BI are intended to represent TX-100 and SL-11W detergent micelles, respectively, while the spherical structures in AII are intended to represent mixed micelles that form during membrane solubilization. In BII, membrane budding is driven by the intercalation of SL-11W molecules into the lipid bilayer, which causes local changes in spontaneous curvature.

Article Snippet: Triton X-100 (TX-100) and 1-pyrenecarboxaldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Simulsol SL 11W (SL-11W) was obtained from Seppic Inc. (Fairfield, NJ, USA).

Techniques: QCM-D, Membrane